u6 expression vectors  (Addgene inc)

Journal: bioRxiv

Article Title: ZNF865 (BLST) Regulates Human Cell Senescence and DNA Damage

doi: 10.1101/2025.06.13.659603

Figure Lengend Snippet: a Structure of the human ZNF865 gene. b ZNF865 expression across different human tissue sources. nTPM = normalized transcripts per million. c dCas9-VPR CRISPRa system and d plasmid maps for lentiviral delivery targeting ZNF865 or NTC. e MA plots from RNA-seq on ZNF865 upregulated HEK293 cells, f ASCs and g human dhNPCs. h Volcano plots showing the top common molecular process affected by ZNF865 upregulation across HEK293 cells, i ASCs and j human dhNPCs using the KEGG 2021 Human Pathway. NTC is non-target control .

Article Snippet: gRNAs, and a non-target that does not target the human genome, were synthesized, annealed, phosphorylated, and ligated into individual U6-gRNA-UbC-DsRed-P2A-Bsr (upregulation gRNA) lentiviral upregulation expression vectors (Addgene, #83919) or KRAB CRISPRi downregulation expression vector pLV-hUbC-dCas9-KRAB-T2A-GFP (Addgene, #71237).

Techniques: Expressing, Plasmid Preparation, RNA Sequencing, Control

Journal: bioRxiv

Article Title: ZNF865 (BLST) Regulates Human Cell Senescence and DNA Damage

doi: 10.1101/2025.06.13.659603

Figure Lengend Snippet:

Article Snippet: gRNAs, and a non-target that does not target the human genome, were synthesized, annealed, phosphorylated, and ligated into individual U6-gRNA-UbC-DsRed-P2A-Bsr (upregulation gRNA) lentiviral upregulation expression vectors (Addgene, #83919) or KRAB CRISPRi downregulation expression vector pLV-hUbC-dCas9-KRAB-T2A-GFP (Addgene, #71237).

Techniques: CRISPR, Control